There are many situations in which a homebrewer might need to propagate yeast using more than one step-up – for example, building up from a bottle of commercial beer, or reviving a smack pack that’s several months old. And while I love Mr. Malty’s Pitching Rate Calculator™, doing two-stage starters with it can be frustrating.

One simple alternative is to use Wyeastâ€™s Pitch Rate and Growth Calculator. It does have some limitations: it works only in gallons, doesn’t feature a viability calculator (although viability can be input manually as partial smack packs), gives results in fairly useless intermediate units (millions/mL) as opposed to cell count or pitching rate (millions/mL-°P), and does not allow for decanting the starter. So in my opinion the best option is to trick the MrMalty calculator into doing it. Here’s how:

- Set your production date or viability, then play with the gravity and/or volume fields until the output matches the volume of your first stage.
- Note the number of cells that result.
- Turn off the automatic viability calculation and enter that number for the viability.
- Enter your actual gravity and volume, and the calculator will tell you the volume of the second stage.
- Verify that the volumes are realistic and adjust the first-stage volume if needed. Ideally, you want to at least double the volume at each step.

For example, let’s say I want to build a three-month-old pack of Wyeast 2206 into enough yeast for a 21 L (5.5 gallon) batch of 20°P (1.084) doppelbock. The required cell count is:

(21 L)(20°P)(1.5 billion/L-°P) = 630 billion. And the pack’s viability is:

0.75^3 = 0.422, or about 42 billion cells.

So I need to increase the cell count by about a factor of 15. This is clearly not possible with a single starter. So I’ll start with a 3 L first stage. Using “intermittent shaking” with a viability of 42%, volume of 5.5 gallons, and gravity of 1.024, the calculator predicts 189 billion cells total. I then set the viability to 189% and the gravity to 1.084, and the calculator tells me that the second stage will need to have a volume of about 6.7 L. Clearly this is one of those situations in which I should be brewing a 2-gallon batch of light lager as a “starter”.

[...] count you need. Getting two-stage numbers out of MrMalty gets a little tricky, but it can be done: Two-Stage Starter Calculations __________________ Beer is like porn. You can buy it, but it's more fun to make your own. [...]

[...] *might* have time to do a two-stage starter, although you won't have time to decant either way. Personally I'd push back the brew date. [...]

[...] the OP. If you are dead set on stepping up, here is a good way to use mr malty to do that. http://seanterrill.com/2010/03/08/two-stage-starter-calculations/ If you are saving some of those from the first batch, youll have to approximate the number. Like, [...]

[...] Has anybody tried this? http://seanterrill.com/2010/03/08/two-stage-starter-calculations/ [...]

[...] 750 mL, then 1.5 L is going to get you to about the same cell count as a 5 L single-stage. http://seanterrill.com/2010/03/08/two-stage-starter-calculations/ __________________ http://seanterrill.com/category/brewing/ [...]

[...] this: Read a lot about how to use Mr. Malty to figure out starter steps, and I finally came across this article which lays out quite nicely how to "trick" the calculator into working for you. I think [...]

Just found your site, and have been browsing around some. Lots of interesting and helpful info, thank you. I recently delved into this problem of stepped starter calculations myself. I put together a web application which you might find useful.

http://www.yeastcalc.summitwoodwork.com Thank you for your contribution to the home brewing community.

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